• Advanced Strategies with EZ Cap™ EGFP mRNA (5-moUTP) for ...

  2025-09-22

  Explore how EZ Cap™ EGFP mRNA (5-moUTP) leverages Cap 1 capping, 5-moUTP modification, and poly(A) tailing to improve mRNA stability, translation efficiency, and immune evasion, supporting robust gene expression and in vivo imaging. Thi

• Trichostatin A (TSA): HDAC Inhibition and Epigenetic Modu...

  2025-09-19

  Trichostatin A (TSA), a potent histone deacetylase inhibitor, is pivotal in epigenetic research and cancer biology. This article explores TSA’s mechanistic role in modulating chromatin structure, its applications in organoid models and breast cancer studies, and its utility as an HDAC inhibitor for epigenetic regulation.

• Polyadenylation Strategies: HyperScribe™ Poly (A) Tailing...

  2025-09-18

  Discover how the HyperScribe™ Poly (A) Tailing Kit enables precise polyadenylation of RNA transcripts, enhancing mRNA stability and translation efficiency for transfection and microinjection experiments. This article explores mechanistic insights, best practices, and emerging applications in post-transcriptional RNA processing.

• The rank order of agonist

  2025-03-03

  

  The rank order of agonist potency in turkey cardiac chambers demonstrated the predominant presence of the β-adrenergic receptor subtype, in line with the receptor classification by . However, a previous radioligand binding study in the left ventricles of 7-week old turkeys reported the existence of

• Among all the compounds the novel L

  2025-03-03

  

  Among all the compounds, the novel L-748,337 derivative (23d) showed the potent human β3-AR antagonist activity and high lipolysis inhibitory activity in vitro. The Gallein structure 23d displayed 23-fold more potent β3-AR antagonist activity (EC50 = 0.5117 nM) than that of L-748,337 (EC50 = 11.91 n

• The importance of insulin in controlling

  2025-03-03

  

  The importance of insulin in controlling lipolysis and fat mass is further emphasized by mouse models with alterations in the insulin signaling pathway of adipocytes. For example, mice with a tamoxifen-inducible knockout of the insulin receptor in mature adipocytes reveal a substantial decrease of a

• Our co IP data show that HT A

  2025-03-03

  

  Our co-IP data show that 5HT1A or 5HT2A receptors do not heterodimerize with mGluR1α receptors. Given that both 5-HT and mGlu receptors are GPCRs and produce anxiolytic effects, a degree of cooperativity via a functional cross-talk may still exist between these receptors [11]. In addition to the fun

• The assay performance was estimated using Z factors

  2025-03-03

  

  The assay performance was estimated using Z′-factors (plotted in Fig. 2A) according to Zhang et al. calculated for each plate comparing positive (in the absence of an inhibitor) and negative controls (in the presence of an AdK inhibitor). The mean Z′-factor±SD was determined to be 0.7±0.1, indicativ

• br Conflict of interest br Acknowledgments

  2025-03-03

  

  Conflict of interest Acknowledgments This work was supported by research grant [PR26/20326] from Santander Bank/UCM. The authors would like to thank Miguel Capo, Professor of Toxicology from the Universidad Complutense de Madrid, for his counseling during the preparation of the present work.

• For both the hydroxylation and lyase reactions the heme

  2025-03-03

  

  For both, the 17α-hydroxylation and 17, 20-lyase reactions, the heme iron center of P450 17A1 receives electron reducing equivalents from CPR. Both reactions require one pair of electrons and molecular oxygen for the conversion of the substrate into product in each catalytic cycle. However, the 17,2

• br Acknowledgements Authors wish to thank Sabanci University

  2025-03-03

  

  Acknowledgements Authors wish to thank Sabanci University (Turkey), Tubitak (Cost Eu-Ros, 113Z463), and the University of Turin (Italy) for supporting this work. Beyza Vurusaner is supported by Sabanci University Post-doctoral research scholarship. Introduction Endometriosis is defined as the

• The LO has so far only

  2025-03-01

  

  The 15-LO-1 has so far only been found in humans and rabbit reticulocytes [3], [20] and the rabbit 15-LO-1 protein demonstrate 81% identity to the human 15-LO-1. However, rabbit monocytes express an enzyme with mainly 12-LO activity [20], [21]. The high degree of sequence conservation between these

• br Material and methods br Results br Discussion

  2025-03-01

  

  Material and methods Results Discussion This study explores the hypothesis that ASK1, via transcriptional upregulation by E2F1, molecularly defines AT that supports a dys-metabolic obese phenotype in humans. We demonstrate associations between increased visceral-AT ASK1 expression and multi

• This study evaluated the anti tumor influences

  2025-03-01

  

  This study evaluated the anti-tumor influences of LA against HepG2 N3-kethoxal intracellular RNA labeling in vivro, and investigated the molecular mechanisms of inducing apoptosis. Overall, our studies suggested that LA is a promising anti-cancer drug and a possible novel therapeutic agent directed

• br Experimental and computation Standard samples of

  2025-03-01

  

  Experimental and computation Standard samples of artemisinin (1) and artemether (2) were purchased from China National Institutes for Food and Drug Control. Their ECD spectra, at a concentration of 1.0–5.0mg/mL in ethanol, were recorded in a quartz cuvette of 1mm optical path length using a Jasco

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